A LACTOBACILLUS ASSAY METHOD FOR p-AMINOBENZOIC ACID

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A MICROBIOLOGICAL ASSAY METHOD FOR p-AMINOBENZOIC ACID

Since the eshablishment of p-aminobenzoic acid as a member of the B vitamin group, a considerable interest has been shown in methods of determination in natural materials. Since known chemical methods are not sufficiently sensitive, it became evident that microbiological tests should be the most practicable. The organism Clostridium acetobutylicum has been used (I) but no general assay procedur...

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Separation of p-Aminobenzoic Acid Derivatives

According to the literature, p-aminobenzoic acid (PAB) is excreted in the urine mostly as the free acid and its N-acetyl derivative; the remaining part is eliminated conjugated with glucuronic acid and as p-aminohippuric acid (PAH) (Ellinger & Hensel, 1914'; Hensel, 1914; Cohen & McGilvery, 1946; Salassa, Bollman & Dry, 1948). There is no agreement concerning the formation of PAH. Thus, Beyer, ...

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THE ISOLATION OF p-AMINOBENZOIC ACID FROM YEAST

The present investigation is primarily concerned with the isolation of p-aminobenzoic acid from yeast. The presence of this substance in yeast was adumbrated by Woods (1) who made the important discovery that. this aromatic amino acid possesses the unique propcity of antagonizing the bsctcriostat~ic action of sulfanilamidt? and that the component. of yeast extracts which exerts a similar antisu...

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Metabolic conjugations of p-aminobenzoic acid in the rat.

The acetylation of p-aminobenzoic acid (PAB) (2) and of sulfanilamide (3) by the rat has been shown to depend upon an adequate supply of pantothenic acid, a constituent of the coenzyme A molecule (4). In addition the extent of acetylation is decreased by giving large amounts of PAB with reference to the size of the animal (5, 6) and to a smaller extent by a deficiency of riboflavin (5). The pre...

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INHIBITION OF ESCHERICHIA COLI BY p-AMINOBENZOIC ACID AND ITS REVERSAL BY p-HYDROXYBENZOIC ACID

p-Aminobenzoic acid (PABA) exerts three metabolic effects on E. coli: it acts as a normal vitamin at low concentrations, as a source of another vitamin, p-hydroxybenzoic acid (POB), at moderate concentrations, and as a growth inhibitor at high concentrations (150 to 1600 microg./ml.). The inhibition is competitively reversed by POB in 1/100 the concentration of PABA. The inhibition is also reve...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1942

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)44964-2